Equipment Needs 

While there are many ways to equip a teaching laboratory for an MCC CURE, The following list of resources is intended as a guide to the optimal equipment and instrumentation that should be accessible to faculty and students in an MCC CURE. For specific Details see the appropriate Protocols

General Lab Equipment

Micropipettors: 2, 10, 20, 200, 1000 and 5000microliter sizes

Pipet Aids for Bacterial work etc

pH meters

Balances to weigh milligram and gram quantities

Stir plates and vortex mixer

Plasmid purification and manipulation

·         Centrifuge[GL1]  for DNA precipitation - capable of 15,000 - 20,000 x g

·         Agarose horizontal gel electrophoresis

·         PCR Thermocycler

·         UV transluminator or other visualization instrument

·         UV/VIs spectrophotometer for DNA/RNA analysis (nano-drop or other similar instrument) 

Bacterial culture and lysis

·         Shaking incubator minimally capable of room temp.   Preferable to maintain temperature below ambient (~16oF and above ~37oF)

·         37oF Incubator 

·         Cell lysis equipment (sonnication , french press, nitrogen cavitation)

·         MicroCentrifuge

·         Spectrophotometer (to detect cell culture density at 600 nm) 

Protein Purification

·         Spectrophotometer or plate reader for protein assay

·         SDS PAGE Electrophoresis

·         Low Speed centrifuge for conducting batch purification 

MDH Enzyme Assay

·         Access to Spectrophotometers or a microplate reader able to measure 340 nm.