The MCC Journal Club is for anyone interested in participating in discussion of important papers relating to structure-function relationships in Malate Dehydrogenases.
The Journal Club meets on the second Thursday of each Month and will discuss the primary paper assigned for the topic together with snippets of related papers. Topics to be discussed and links to the appropriate papers can be found on the schedule below together with the zoom link for the session. After each session the powerpoint from the session will be linked to this page.
Each Session a Primary Paper will be presented and discussed together with relevant snippets from secondary papers
All primary papers are freely accessible via PubMed- links given.
See the Topics and papers here- password protected- contact Ellis Bell, jbell@sandiego.edu to obtain the password
Zoom Link to Join:
MOLECULAR Life Sciences is inviting you to a scheduled Zoom meeting.
Topic: MCC Journal Club-MOLECULAR Life Sciences' Zoom Meeting
Time: Oct 12, 2023 05:00 PM Pacific Time (US and Canada)
Every month on the Second Thu, 10 occurrence(s)
Please download and import the following iCalendar (.ics) files to your calendar system.
Monthly: https://us02web.zoom.us/meeting/tZAkc-iorT8rG9Gzzn93-9vruHh-rWskcy6V/ics?icsToken=98tyKuGspz8rGteRsRiCRpwIHY-gd_Tztilcjfp6hgXrWnMeOxXkI8pKf6BIA9PT
Join Zoom Meeting
https://us02web.zoom.us/j/84941034115?pwd=dGV3NHhTWXFKYmw0dGxSSnpjN09TUT09
Meeting ID: 849 4103 4115
Passcode: 754599
Thursday October 12th
Mechanism of Catalysis,
Primary Paper: J Biol Chem, 258, No. 1, Issue of January IO, pp. 472-482: https://pubmed.ncbi.nlm.nih.gov/6848515/
The Presence of a Histidine-Aspartic Acid Pair in the Active Site of
2-Hydroxyacid Dehydrogenases
Secondary Papers:
https://pubmed.ncbi.nlm.nih.gov/31361/
https://pubmed.ncbi.nlm.nih.gov/9125501/
https://pubmed.ncbi.nlm.nih.gov/8665917/
https://pubmed.ncbi.nlm.nih.gov/6959107/
Thursday November 9th
Kinetic Mechanism,
Primary Paper: J Biol Chem, 1968 May 10;243(9):2413-23: https://pubmed.ncbi.nlm.nih.gov/4296842/
Kinetic Studies on the Mechanism of the Malate Dehydrogenase Reaction
Secondary Papers:
https://pubmed.ncbi.nlm.nih.gov/25606688/
https://pubmed.ncbi.nlm.nih.gov/4307999/
Thursday December 14th
Metabolon Formation
Primary Paper: Angew Chem Int Ed Engl. 2015 Feb 2;54(6):1851-4. doi: 10.1002/anie.201409336. Epub 2014 Dec 23. PMID: 25537779.: https://pubmed.ncbi.nlm.nih.gov/25537779/
Krebs Cycle Metabolon: Structural Evidence of Substrate Channeling
Revealed by Cross-Linking and Mass Spectrometry**
Secondary Papers:
https://pubmed.ncbi.nlm.nih.gov/27556423/
https://pubmed.ncbi.nlm.nih.gov/8611153/
https://pubmed.ncbi.nlm.nih.gov/37102132/
https://pubmed.ncbi.nlm.nih.gov/34548590/
https://pubmed.ncbi.nlm.nih.gov/29094409/
https://pubmed.ncbi.nlm.nih.gov/24325460/
Thursday January 11th
Cofactor Specificity,
Primary Paper: J Biol Chem. 1993 Mar 5;268(7):4656-60. PMID: 8444839.
https://pubmed.ncbi.nlm.nih.gov/8444839/
Alteration of coenzyme specificity of malate dehydrogenase from Thermus flavus by site-directed mutagenesis.
Secondary Papers:
https://pubmed.ncbi.nlm.nih.gov/7832772/
https://pubmed.ncbi.nlm.nih.gov/30638660/
https://pubmed.ncbi.nlm.nih.gov/19772885/
https://pubmed.ncbi.nlm.nih.gov/7833810/
Thursday February 8th
Oligomeric Structure,
Primary Paper: Protein Sci. 1994 Nov;3(11):2023-32. doi: 10.1002/pro.5560031115. PMID: 7703849; PMCID: PMC2142640.: https://pubmed.ncbi.nlm.nih.gov/7703849/
Engineering the quaternary structure of an enzyme: construction and analysis of a monomeric form of malate dehydrogenase from Escherichia coli.
Secondary Papers
https://pubmed.ncbi.nlm.nih.gov/3114237/
https://pubmed.ncbi.nlm.nih.gov/31301348/
https://pubmed.ncbi.nlm.nih.gov/10825536/
Thursday March 14th
Interface and Allostery,
Primary: J Biol Chem. 1982 Nov 25;257(22):13233-9. PMID: 7142142.: https://pubmed.ncbi.nlm.nih.gov/7142142/
Regulation of mitochondrial malate dehydrogenase. Evidence for an allosteric citrate-binding site.
Secondary:
https://pubmed.ncbi.nlm.nih.gov/8251495/
https://pubmed.ncbi.nlm.nih.gov/4299102/
https://pubmed.ncbi.nlm.nih.gov/3985618/
Thursday April 11th
MDH and Drug Design,
Primary Paper: Commun Biol. 2021 Aug 10;4(1):949. doi: 10.1038/s42003-021-02442-1. PMID: 34376783; PMCID: PMC8355244.: https://pubmed.ncbi.nlm.nih.gov/34376783/
A fragment-based approach identifies an allosteric pocket that impacts malate dehydrogenase activity
Secondary Papers
https://pubmed.ncbi.nlm.nih.gov/29694407/
https://pubmed.ncbi.nlm.nih.gov/8615849/
https://pubmed.ncbi.nlm.nih.gov/30108105/
Thursday May 9th
Loop and Substrate Specificity,
Primary Paper: Nat Chem Biol. 2017 May;13(5):494-500. doi: 10.1038/nchembio.2307. Epub 2017 Mar 6. PMID: 28263965; PMCID: PMC5516644.: https://pubmed.ncbi.nlm.nih.gov/28263965/
L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH
Secondary Papers
https://pubmed.ncbi.nlm.nih.gov/10075524/
https://pubmed.ncbi.nlm.nih.gov/27510037/
https://pubmed.ncbi.nlm.nih.gov/7574693/
https://pubmed.ncbi.nlm.nih.gov/34850194/
Thursday June 13th
PTMs and MDH
Primary Paper: Plant J. 2022 Jan;109(1):92-111. doi: 10.1111/tpj.15556. Epub 2021 Nov 10. PMID: 34713507.: https://pubmed.ncbi.nlm.nih.gov/34713507/
Acetylation of conserved lysines fine-tunes mitochondrial malate dehydrogenase activity in land plants
Secondary Papers
https://pubmed.ncbi.nlm.nih.gov/7986072/
https://pubmed.ncbi.nlm.nih.gov/28366830/
https://pubmed.ncbi.nlm.nih.gov/35879546/